LOCUS pLKO_AS3w.tRFP-C 9292 bp DNA circular 30-OCT-2008 SOURCE ORGANISM COMMENT 1. Sequences from nt1-nt 636 and nt4010 confirmed (10/28/2008). COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|496658516| COMMENT VNTDBDATE|497521305| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_AS3w.tRFP-C| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CWidget 12 (0 0) 1 2 0 0 Nil -918 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1633772391 COMMENT (CStringList) 1667721059 0 (CObList))) FEATURES Location/Qualifiers CDS 5395..6252 /vntifkey="4" /label=Amp misc_feature 6456..6996 /vntifkey="21" /label=Ori LTR 4000..4244 /vntifkey="19" /label=HIV\3'-LTR LTR 7710..7890 /vntifkey="19" /label=HIV\5'-LTR misc_feature 8566..8770 /vntifkey="21" /label=RRE misc_feature 8001..8045 /vntifkey="21" /label=Psi\sequence promoter 7481..7709 /vntifkey="29" /label=RSV\Promoter misc_feature 4306..4436 /vntifkey="21" /label=SV40\PolyA promoter 5296..5394 /vntifkey="30" /label=bla\promoter promoter 240..599 /vntifkey="29" /label=CMVieEnh misc_feature 600..1946 /vntifkey="21" /label=Beta-Actinp misc_feature 6..123 /vntifkey="21" /label=cPPT promoter 240..1946 /vntifkey="29" /label=CAG\promoter misc_feature 2014..2616 /vntifkey="21" /label=IRES1 CDS 2614..3309 /vntifkey="4" /label=turboRFP-C misc_feature 3325..3913 /vntifkey="21" /label=WPRE BASE COUNT 2212 a 2342 c 2554 g 2184 t ORIGIN 1 tcgagtttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac 61 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat 121 tttactagtg aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga 181 tgtacgggcc agaaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttct 241 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 421 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 481 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 541 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 601 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc 661 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg 721 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg 781 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg 841 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc 901 gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 961 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 1021 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg 1081 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg 1141 ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 1201 tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg 1261 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg 1321 tgggcgcggc ggtcgggctg taaccccccc ctgcaacccc ctccccgagt tgctgagcac 1381 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc 1441 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc 1501 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc 1561 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc 1621 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag 1681 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc 1741 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg 1801 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct 1861 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg 1921 ctgtctcatc attttggcaa agaatttaat acgactcact atagggagac ccaagctggc 1981 tagcggcgcg ccgaattcct gcagggttta aactgtgtta agatccgccc ctctccctcc 2041 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 2101 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 2161 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 2221 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 2281 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 2341 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 2401 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 2461 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat 2521 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 2581 tttgaaaaac acgatgataa tatggccaca accatgagcg agctgatcaa ggagaacatg 2641 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag 2701 ggcgaaggca agccctacga gggcacccag accatgaaga tcaaggtggt cgagggcggc 2761 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaagccttc 2821 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca 2881 tgggagagaa tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc 2941 ttccagaacg gctgcatcat ctacaacgtc aagatcaacg gggtgaactt cccatccaac 3001 ggccctgtga tgcagaagaa aacacgcggc tgggaggcca acaccgagat gctgtacccc 3061 gctgacggcg gcctgagagg ccacagccag atggccctga agctcgtggg cgggggctac 3121 ctgcactgct ccttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg 3181 cccggcttcc acttcgtgga ccacagactg gaaagaatca aggaggccga caaagagacc 3241 tacgtcgagc agcacgagat ggctgtggcc aagtactgcg acctccctag caaactgggg 3301 cacagataaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg 3361 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 3421 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 3481 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 3541 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 3601 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 3661 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 3721 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 3781 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 3841 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 3901 gccgcctccc cgcccgggtc gactctagat gtacctttaa gaccaatgac ttacaaggca 3961 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 4021 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 4081 gcctgggagc tctctggcta actagagaac ccactgctta agcctcaata aagcttgcct 4141 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 4201 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 4261 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 4321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 4381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 4441 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 4501 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 4561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 4621 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 4681 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 4741 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 4801 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4861 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4921 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4981 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 5041 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 5101 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 5161 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 5221 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 5281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 5341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 5401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 5461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 5641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 5941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 6001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 6121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 6601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 7021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 7081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 7141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 7201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 7261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 7321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 7381 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 7441 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac 7501 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa 7561 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc 7621 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg 7681 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct 7741 gggagctctc tggctaacta gagaacccac tgcttaagcc tcaataaagc ttgccttgag 7801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 7861 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 7921 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 7981 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 8041 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 8101 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 8161 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 8221 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 8281 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 8341 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 8401 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 8461 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 8521 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 8581 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 8641 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 8701 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 8761 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 8821 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 8881 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 8941 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 9001 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 9061 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 9121 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 9181 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca 9241 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag cc //