LOCUS pLKO_AS2.bsd 7163 bp DNA circular 31-JAN-2008 SOURCE ORGANISM COMMENT 1. Seqences at nt6685 to 1 to 1502 were confirmed. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|472058801| COMMENT VNTDBDATE|472742889| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_AS2.bsd| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS 3266..4123 /vntifkey="4" /label=Amp misc_feature 4327..4867 /vntifkey="21" /label=Ori LTR 1871..2115 /vntifkey="19" /label=HIV\3'-LTR LTR 5581..5761 /vntifkey="19" /label=HIV\5'-LTR misc_feature 6437..6641 /vntifkey="21" /label=RRE misc_feature 5872..5916 /vntifkey="21" /label=Psi\sequence promoter 5352..5580 /vntifkey="29" /label=RSV\Promoter misc_feature 2177..2307 /vntifkey="21" /label=SV40\PolyA promoter 3167..3265 /vntifkey="30" /label=bla\promoter promoter 88..675 /vntifkey="29" /label=CMV\promoter misc_feature 786..1388 /vntifkey="21" /label=EMCVI /note="IRES" misc_feature 1386..1784 /vntifkey="21" /label=Blasticidin BASE COUNT 1896 a 1679 c 1808 g 1780 t ORIGIN 1 tcgaggatcc actagtgaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc 61 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt 121 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 181 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 241 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 301 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt 481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc 541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat 601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 661 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta 721 atacgactca ctatagggag acccaagctg gctagcggcg cgccgaattc ctgcagggtt 781 taaactgtgt taagatccgc ccctctccct ccccccccct aacgttactg gccgaagccg 841 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 901 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 961 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 1021 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 1081 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 1141 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 1201 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 1261 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 1321 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca 1381 caaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta 1441 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg 1501 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac 1561 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga 1621 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg 1681 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg 1741 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaaatgggt cgactctaga 1801 tgtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 1861 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 1921 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 1981 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 2041 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 2101 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 2161 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 2221 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 2281 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 2341 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 2401 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 2461 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 2521 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 2581 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 2641 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 2701 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 2761 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 2821 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 2881 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 2941 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 3001 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 3061 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 3121 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 3181 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3241 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 3301 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 3361 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 3421 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 3481 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 3541 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 3601 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 3661 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 3721 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 3781 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 3841 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 3901 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 3961 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 4021 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 4081 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 4141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 4201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 4261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 4321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 4381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 4441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 4501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 4561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 4621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 4681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 4741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 4801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 4861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 4921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 4981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 5041 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 5101 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 5161 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 5221 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 5281 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 5341 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg 5401 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta 5461 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga 5521 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac 5581 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agagaaccca 5641 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5701 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5761 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 5821 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 5881 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 5941 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 6001 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 6061 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 6121 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 6181 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 6241 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 6301 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 6361 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 6421 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 6481 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 6541 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 6601 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 6661 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 6721 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 6781 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 6841 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 6901 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 6961 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 7021 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 7081 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 7141 cggtatcgat cacgagacta gcc //