LOCUS pLKO_TRC008 9930 bp DNA circular 12-JAN-2009 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|507029119| COMMENT VNTDBDATE|507029119| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_TRC008| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1735357044 COMMENT (CStringList) 544370548 2083664691 (CObList))) FEATURES Location/Qualifiers misc_feature 3988..4794 /vntifkey="21" /label=AMP\r rep_origin complement(4920..5535) /vntifkey="33" /label=pUC\Origin promoter 4..508 /vntifkey="29" /label=hPGK\promoter LTR 5883..6295 /vntifkey="19" /label=5'LTR misc_feature 9761..9878 /vntifkey="21" /label=cPPT rep_origin 3187..3617 /vntifkey="33" /label=F1\ORI misc_feature 6450..6814 /vntifkey="21" /label=gag misc_feature 6960..7201 /vntifkey="21" /label=RRE misc_feature 6346..6483 /vntifkey="21" /label=psi promoter 7599..7850 /vntifkey="29" /label=hU6\(-250) misc_marker 7851..9719 /vntifkey="22" /label=1.9\kb\Stuffer LTR 2398..2633 /vntifkey="19" /label=3'\LTR\SIN misc_RNA 1739..2327 /vntifkey="53" /label=WPRE misc_feature 921..1019 /vntifkey="21" /label=GSG-F2A misc_feature 1026..1724 /vntifkey="21" /label=turboGFP misc_feature 519..914 /invitrogen="1050000" /vntifkey="21" /label=Bsd(R) BASE COUNT 2589 a 2344 c 2307 g 2690 t ORIGIN 1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaccat ggccaagcct ttgtctcaag 541 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag 601 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg 661 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg 721 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga 781 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag 841 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt 901 atgtgtggga gggcctgcag ggatctggcg aagccagaca caaacagaaa attgtgaaac 961 agactttgaa ttttgacctt ctcaagttgg cgggagacgt ggagtccaac ccagggcccg 1021 ctagcatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg 1081 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccgagcagg 1141 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc 1201 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga 1261 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg 1321 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg 1381 gcgacttcaa ggtgatgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga 1441 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gatctggatg 1501 gcagcttcac ccgcaccttc agcctgcgcg acggcggcta ctacagctcc gtggtggaca 1561 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt 1621 tcgccttccg ccgcgtggag gaggatcaca gcaacaccga gctgggcatc gtggagtacc 1681 agcacgcctt caagaccccg gatgcagatg ccggtgaaga ataacgcgtt aagtcgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 1801 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 1861 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 1921 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 1981 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 2041 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 2101 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 2161 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 2221 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 2281 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga 2341 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 2401 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc 2461 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 2521 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 2581 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat 2641 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 2701 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 2761 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 2821 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 2881 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 2941 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 3001 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 3061 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 3121 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 3181 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 3241 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 3301 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 3361 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 3421 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 3481 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 3541 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 3601 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 3661 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 3721 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 3781 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 3841 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 3901 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 3961 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 4021 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 4081 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 4141 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 4201 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 4261 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 4321 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 4381 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 4441 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 4501 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 4561 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 4621 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 4681 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 4741 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 4801 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 4861 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 4921 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 4981 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5041 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5101 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 5161 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5221 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5281 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5341 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5401 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 5461 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 5521 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 5581 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 5641 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 5701 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 5761 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 5821 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa 5881 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc 5941 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 6001 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg 6061 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc 6121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 6301 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 6361 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 6421 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 6481 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 6541 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 6601 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 6661 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 6721 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 6781 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 6841 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 6901 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 6961 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 7021 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 7081 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 7141 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 7201 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 7261 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 7321 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 7381 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 7441 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 7501 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 7561 cgtttcagac ccacctccca accccgaggg gacccagaga gggcctattt cccatgattc 7621 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg 7681 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt 7741 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt 7801 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggtaccggt gtgttgtaaa 7861 tgagcacaca aaatacacat gctaaaatat tatattctat gacctttata aaatcaacca 7921 aaatcttctt tttaataact ttagtatcaa taattagaat ttttatgttc ctttttgcaa 7981 acttttaata aaaatgagca aaataaaaaa acgctagttt tagtaactcg cgttgttttc 8041 ttcaccttta ataatagcta ctccaccact tgttcctaag cggtcagctc ctgcttcaat 8101 cattttttga gcatcttcaa atgttctaac tccaccagct gctttaacta aagcattgtc 8161 tttaacaact gacttcatta gtttaacatc ttcaaatgtt gcacctgatt ttgaaaatcc 8221 tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct atttcacaag ctttcatgat 8281 ttcttctttt gttaataaac aattttccat aatacattta acaacatgtg atccagctgc 8341 tttttttaca gctttcatgt cttctaaaac taattcataa tttttgtctt ttaatgcacc 8401 aatatttaat accatatcaa tttctgttgc accatcttta attgcttcag aaacttcgaa 8461 tgcttttgta gctgttgtgc atgcacctag aggaaaacct acaacatttg ttattcctac 8521 atttgtgcct tttaataatt ctttacaata gcttgttcaa tatgaattaa cacaaactgt 8581 tgcaaaatca aattcaattg cttcatcaca taattgttta atttcagctt tcgtagcatc 8641 ttgttttaat aatgtgtgat ctatatattt gtttagtttc attttttctc ctatatattc 8701 atttttaatt ttaattcttt aataatttcg tctactttaa ctttagcgtt ttgaacagat 8761 tcaccaacac ctataaaata aatttttagt ttaggttcag ttccacttgg gcgaacagca 8821 aatcatgact tatcttctaa ataaaatttt agtaagtctt gtcctggcat attatacatt 8881 ccatcgatgt agtcttcaac attaacaact ttaagtccag caatttgagt taagggtgtt 8941 gctctcaatg atttcattaa tggttcaatt tttaatttct tttcttctgg tttaaaattc 9001 aagtttaaag tgaaagtgta atatgcaccc atttctttaa ataaatcttc taaatagtct 9061 actaatgttt tattttgttt tttataaaat caagcagcct ctgctattaa tatagaagct 9121 tgtattccat ctttatctct agctgagtca tcaattacat atccataact ttcttcataa 9181 gcaaaaacaa aatttaatcc gttatcttct tctttagcaa tttctctacc cattcattta 9241 aatccagtta aagtttttac aatattaact ccatattttt catgagcgat tctatcaccc 9301 aaatcacttg ttacaaaact tgaatataga gccggatttt ttggaatgct atttaagcgt 9361 tttagatttg ataattttca atcaattaaa attggtcctg tttgatttcc atctaatctt 9421 acaaaatgac catcatgttt tattgccatt ccaaatctgt cagcatctgg gtcattcata 9481 ataataatat ctgcatcatg tttaatacca tattcaagcg gtatttttca tgcaggatca 9541 aattctggat ttggatttac aacattttta aatgtttcat cttcaaatgc atgctcttca 9601 acctcaataa cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt 9661 ccattaactg cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtag 9721 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 9781 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 9841 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 9901 gagatccact ttggcgccgg ctcgaggggg //