LOCUS pLKO_AS1010\(PeG 10724 bp DNA circular 17-APR-2017 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|670933197| COMMENT VNTDBDATE|-11724008| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_AS1010 (PeGFP-I2-Puro)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(@S)" 5 -9.3135 COMMENT 10.454 0 -10 5.41155 0.932452 #209#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #211=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1182 100) COMMENT (CObjectList) (CObjectList)) COMMENT #212=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1078 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #213=(AnlzFontColorItem 0 10723 33554432)) 1 (CObList)) COMMENT (CObList) 760234312 (CStringList) 1300001381 673198897 (CObList))) FEATURES Location/Qualifiers misc_feature 4782..5588 /vntifkey="21" /label=AMP\r rep_origin complement(5714..6329) /vntifkey="33" /label=pUC\Origin promoter 4..508 /vntifkey="29" /label=hPGK\promoter LTR 6677..7089 /vntifkey="19" /label=5'LTR misc_feature 10555..10672 /vntifkey="21" /label=cPPT rep_origin 3981..4411 /vntifkey="33" /label=F1\ORI misc_feature 7244..7608 /vntifkey="21" /label=gag misc_feature 7754..7995 /vntifkey="21" /label=RRE misc_feature 7140..7277 /vntifkey="21" /label=psi promoter 8393..8644 /vntifkey="29" /label=hU6\(-250) misc_marker 8645..10513 /vntifkey="22" /label=1.9\kb\Stuffer LTR 3192..3427 /vntifkey="19" /label=3'\LTR\SIN misc_RNA 2533..3121 /vntifkey="53" /label=WPRE CDS 531..1250 /vntifkey="4" /label=eGFP misc_feature 1322..1918 /vntifkey="21" /label=IRES CDS 1919..2518 /vntifkey="4" /label=PAC BASE COUNT 2738 a 2634 c 2535 g 2817 t ORIGIN 1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaatac tgtggccagc atggtgagca 541 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 601 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 661 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 721 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 781 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 841 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 901 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 961 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 1021 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 1081 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 1141 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 1201 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa aattcctgca 1261 gggtttaaac tagactcgag cggccgccac tgtgcacgat gatccgagct cggtaccaag 1321 cttaagatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 1381 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 1441 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 1501 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 1561 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 1621 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 1681 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 1741 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atccgatctg 1801 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 1861 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat 1921 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 1981 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 2041 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 2101 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 2161 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 2221 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 2281 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 2341 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 2401 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 2461 cgtcaaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg 2521 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 2581 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 2641 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 2701 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 2761 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 2821 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 2881 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 2941 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 3001 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 3061 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 3121 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 3181 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt 3241 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 3301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 3361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 3421 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 3481 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 3541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 3601 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 3661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 3721 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 3781 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac 3841 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 3901 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 3961 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 4021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 4081 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 4141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 4201 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 4261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 4321 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 4381 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 4441 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4561 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 4621 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4681 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4741 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4801 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4861 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 4921 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 4981 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 5041 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 5101 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 5161 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 5221 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 5281 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 5341 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 5401 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 5461 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5761 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6001 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 6361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 6421 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 6481 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 6541 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 6601 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa 6661 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 6721 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 6781 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 6841 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 6901 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6961 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7021 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7081 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 7141 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 7201 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 7261 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 7321 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 7381 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 7441 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 7501 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 7561 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg 7621 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat 7681 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag 7741 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg 7801 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca 7861 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg 7921 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca 7981 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa 8041 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg 8101 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa 8161 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa 8221 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg 8281 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg 8341 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct 8401 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag 8461 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata 8521 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac 8581 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaggtac 8641 cggtgtgttg taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt 8701 tataaaatca accaaaatct tctttttaat aactttagta tcaataatta gaatttttat 8761 gttccttttt gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa 8821 ctcgcgttgt tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca 8881 gctcctgctt caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta 8941 actaaagcat tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct 9001 gattttgaaa atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca 9061 caagctttca tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca 9121 tgtgatccag ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg 9181 tcttttaatg caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct 9241 tcagaaactt cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca 9301 tttgttattc ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa 9361 ttaacacaaa ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca 9421 gctttcgtag catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt 9481 tctcctatat attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag 9541 cgttttgaac agattcacca acacctataa aataaatttt tagtttaggt tcagttccac 9601 ttgggcgaac agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg 9661 gcatattata cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt 9721 gagttaaggg tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt 9781 ctggtttaaa attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat 9841 cttctaaata gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta 9901 ttaatataga agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat 9961 aactttcttc ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc 10021 tacccattca tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag 10081 cgattctatc acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa 10141 tgctatttaa gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat 10201 ttccatctaa tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat 10261 ctgggtcatt cataataata atatctgcat catgtttaat accatattca agcggtattt 10321 ttcatgcagg atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa 10381 atgcatgctc ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa 10441 atttagttcc tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt 10501 gctctttttt gtagaattct agatcttgag acaaatggca gtattcatcc acaattttaa 10561 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 10621 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 10681 ttacagggac agcagagatc cactttggcg ccggctcgag gggg //