LOCUS C6-7-20\pLKO_AS1 9844 bp DNA circular 22-AUG-2025 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT Called pLKO.GC33 Maxiprep sequence verified Job #11818 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-16114590| COMMENT VNTDBDATE|-16114595| COMMENT LSOWNER| COMMENT VNTNAME|C6-7-20 pLKO_AS1009 (Pbsd-L-tRFP)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(LOGPEN 0 8 10053171) 8 1 0.133825 1.9 COMMENT 0.082322 1) COMMENT #212=(CLabel (CWidget 0 (0 0) 1 2 0 0 #128# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (9635)" "@N (@S)" 5 -8.96504 COMMENT 10.454 0 -10 5.06188 0.965754 #211#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #213=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1601 100) COMMENT (CObjectList) (CObjectList)) COMMENT #214=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -346 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 16 (CObjectList) COMMENT (CObList #215=(AnlzFontColorItem 0 9843 33554432)) 1 (CObList)) COMMENT (CObList) 1735357044 (CStringList) 544370548 2083664691 (CObList))) FEATURES Location/Qualifiers misc_feature 3902..4708 /vntifkey="21" /label=AMP\r rep_origin complement(4834..5449) /vntifkey="33" /label=pUC\Ori promoter 4..508 /vntifkey="29" /label=hPGK LTR 2312..2547 /vntifkey="19" /label=HIV\sin\3'\LTR rep_origin 3101..3531 /vntifkey="33" /label=F1\Ori misc_feature 6874..7115 /vntifkey="21" /label=RRE misc_RNA 1653..2241 /vntifkey="53" /label=WPRE CDS 519..914 /invitrogen="1050000" /vntifkey="4" /label=Bsd(R) CDS 942..1637 /vntifkey="4" /label=turboRFP-C misc_marker 7765..9633 /vntifkey="22" /label=Stuffer promoter 7513..7764 /vntifkey="29" /label=hU6\promoterr misc_feature 915..941 /vntifkey="21" /label=Linker promoter 5799..6027 /vntifkey="29" /label=RSV\Promoter LTR 6028..6208 /vntifkey="19" /label=HIV\5'-LTR misc_feature 6260..6397 /vntifkey="21" /label=psi misc_feature 6364..6728 /vntifkey="21" /label=gag misc_feature 9675..9792 /vntifkey="21" /label=cPPT BASE COUNT 2596 a 2304 c 2264 g 2680 t ORIGIN 1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaccat ggccaagcct ttgtctcaag 541 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag 601 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg 661 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg 721 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga 781 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag 841 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt 901 atgtgtggga gggcctgcac ggcagcggca gcggctctag catgagcgag ctgatcaagg 961 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca 1021 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc aaggtggtcg 1081 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca 1141 aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg 1201 gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc gctacccagg 1261 acaccagctt ccagaacggc tgcatcatct acaacgtcaa gatcaacggg gtgaacttcc 1321 catccaacgg ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac accgagatgc 1381 tgtaccccgc tgacggcggc ctgagaggcc acagccagat ggccctgaag ctcgtgggcg 1441 ggggctacct gcactgctcc ttcaagacca catacagatc caagaaaccc gctaagaacc 1501 tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag gaggccgaca 1561 aagagaccta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac ctccctagca 1621 aactggggca cagataaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg 1681 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 1741 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 1801 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 1861 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 1921 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 1981 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 2041 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 2101 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 2161 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 2221 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga 2281 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 2341 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 2401 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 2461 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 2521 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca 2581 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 2641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 2701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 2761 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 2821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 2881 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc 2941 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg 3001 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 3061 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 3121 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 3181 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 3241 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 3301 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 3361 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 3421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 3481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 3541 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 3601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 3721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 3781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 3841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 3901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 3961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 4021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 4081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 4141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 4201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 4261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 4321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 4381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 4441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 4501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 4561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 4621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 4681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 4921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 5041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 5101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 5161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 5221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 5281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 5341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 5401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 5461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc 5521 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 5581 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 5641 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 5701 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa 5761 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc 5821 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 5881 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa 5941 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta 6001 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg 6061 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 6121 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 6181 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag 6241 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 6301 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 6361 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 6421 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 6481 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 6541 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 6601 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 6661 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 6721 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat 6781 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga 6841 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga 6901 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta 6961 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat 7021 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa 7081 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc 7141 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat 7201 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc 7261 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat 7321 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta 7381 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata 7441 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg 7501 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag 7561 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat 7621 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa 7681 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc 7741 ttgtggaaag gacgaggtac cggtgtgttg taaatgagca cacaaaatac acatgctaaa 7801 atattatatt ctatgacctt tataaaatca accaaaatct tctttttaat aactttagta 7861 tcaataatta gaatttttat gttccttttt gcaaactttt aataaaaatg agcaaaataa 7921 aaaaacgcta gttttagtaa ctcgcgttgt tttcttcacc tttaataata gctactccac 7981 cacttgttcc taagcggtca gctcctgctt caatcatttt ttgagcatct tcaaatgttc 8041 taactccacc agctgcttta actaaagcat tgtctttaac aactgacttc attagtttaa 8101 catcttcaaa tgttgcacct gattttgaaa atcctgttga tgttttaaca aattctaatc 8161 cagcttcaac agctatttca caagctttca tgatttcttc ttttgttaat aaacaatttt 8221 ccataataca tttaacaaca tgtgatccag ctgctttttt tacagctttc atgtcttcta 8281 aaactaattc ataatttttg tcttttaatg caccaatatt taataccata tcaatttctg 8341 ttgcaccatc tttaattgct tcagaaactt cgaatgcttt tgtagctgtt gtgcatgcac 8401 ctagaggaaa acctacaaca tttgttattc ctacatttgt gccttttaat aattctttac 8461 aatagcttgt tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca attgcttcat 8521 cacataattg tttaatttca gctttcgtag catcttgttt taataatgtg tgatctatat 8581 atttgtttag tttcattttt tctcctatat attcattttt aattttaatt ctttaataat 8641 ttcgtctact ttaactttag cgttttgaac agattcacca acacctataa aataaatttt 8701 tagtttaggt tcagttccac ttgggcgaac agcaaatcat gacttatctt ctaaataaaa 8761 ttttagtaag tcttgtcctg gcatattata cattccatcg atgtagtctt caacattaac 8821 aactttaagt ccagcaattt gagttaaggg tgttgctctc aatgatttca ttaatggttc 8881 aatttttaat ttcttttctt ctggtttaaa attcaagttt aaagtgaaag tgtaatatgc 8941 acccatttct ttaaataaat cttctaaata gtctactaat gttttatttt gttttttata 9001 aaatcaagca gcctctgcta ttaatataga agcttgtatt ccatctttat ctctagctga 9061 gtcatcaatt acatatccat aactttcttc ataagcaaaa acaaaattta atccgttatc 9121 ttcttcttta gcaatttctc tacccattca tttaaatcca gttaaagttt ttacaatatt 9181 aactccatat ttttcatgag cgattctatc acccaaatca cttgttacaa aacttgaata 9241 tagagccgga ttttttggaa tgctatttaa gcgttttaga tttgataatt ttcaatcaat 9301 taaaattggt cctgtttgat ttccatctaa tcttacaaaa tgaccatcat gttttattgc 9361 cattccaaat ctgtcagcat ctgggtcatt cataataata atatctgcat catgtttaat 9421 accatattca agcggtattt ttcatgcagg atcaaattct ggatttggat ttacaacatt 9481 tttaaatgtt tcatcttcaa atgcatgctc ttcaacctca ataacgttat atcctgattc 9541 acgtaatatt tttggggtaa atttagttcc tgttccatta actgcgctaa aaataatttt 9601 taaatctttt ttagcttctt gctctttttt gtagaattct agatcttgag acaaatggca 9661 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 9721 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 9781 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag 9841 gggg //