LOCUS pLKO_TRC017 9530 bp DNA circular 12-JAN-2009 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|507052176| COMMENT VNTDBDATE|507052176| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_TRC017| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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6560..6801 /vntifkey="21" /label=RRE misc_feature 5946..6083 /vntifkey="21" /label=psi promoter 7199..7450 /vntifkey="29" /label=hU6\(-250) misc_feature 7451..9319 /vntifkey="21" /label=1.9\kb\Stuffer LTR 1998..2233 /vntifkey="19" /label=3'\LTR\SIN misc_feature 1339..1927 /vntifkey="21" /label=WPRE misc_feature 519..1314 /gene="neo/kan" /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="ABG47805.1" /db_xref="GI:109900620" /vntifkey="21" /label=neo/kan BASE COUNT 2463 a 2200 c 2208 g 2659 t ORIGIN 1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaccat gattgaacaa gatggattgc 541 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 601 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 661 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 721 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 781 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 841 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 901 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 961 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 1021 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 1081 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 1141 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 1201 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 1261 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc ctgcagggat 1321 aagacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 1381 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 1441 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 1501 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 1561 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 1621 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 1681 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 1741 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 1801 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 1861 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 1921 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 1981 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt 2041 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 2101 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 2161 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 2221 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg 2281 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 2341 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 2401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 2461 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2521 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 2581 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg 2641 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 2701 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 2761 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 2821 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2881 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 2941 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3001 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 3061 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3121 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 3181 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3241 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 3301 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 3361 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 3421 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 3481 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 3541 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 3601 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 3661 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 3721 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 3781 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 3841 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 3901 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 3961 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 4021 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 4081 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 4141 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 4201 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 4261 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 4321 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 4381 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 4441 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 4501 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 4561 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 4621 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 4681 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 4741 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 4801 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 4861 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 4921 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 4981 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 5041 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 5101 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 5161 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 5221 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 5281 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 5341 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 5401 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg 5461 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa 5521 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg 5581 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac 5641 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc 5701 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 5761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 5821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 5881 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag 5941 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga 6001 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag 6061 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca 6121 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga 6181 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag 6241 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca 6301 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag 6361 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag 6421 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt 6481 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga 6541 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac 6601 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt 6661 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac 6721 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga 6781 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc 6841 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat 6901 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc 6961 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt 7021 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt 7081 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag 7141 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga 7201 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat 7261 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa 7321 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat 7381 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg 7441 aggtaccggt gtgttgtaaa tgagcacaca aaatacacat gctaaaatat tatattctat 7501 gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa taattagaat 7561 ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa acgctagttt 7621 tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact tgttcctaag 7681 cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac tccaccagct 7741 gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc ttcaaatgtt 7801 gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct 7861 atttcacaag ctttcatgat ttcttctttt gttaataaac aattttccat aatacattta 7921 acaacatgtg atccagctgc tttttttaca gctttcatgt cttctaaaac taattcataa 7981 tttttgtctt ttaatgcacc aatatttaat accatatcaa tttctgttgc accatcttta 8041 attgcttcag aaacttcgaa tgcttttgta gctgttgtgc atgcacctag aggaaaacct 8101 acaacatttg ttattcctac atttgtgcct tttaataatt ctttacaata gcttgttcaa 8161 tatgaattaa cacaaactgt tgcaaaatca aattcaattg cttcatcaca taattgttta 8221 atttcagctt tcgtagcatc ttgttttaat aatgtgtgat ctatatattt gtttagtttc 8281 attttttctc ctatatattc atttttaatt ttaattcttt aataatttcg tctactttaa 8341 ctttagcgtt ttgaacagat tcaccaacac ctataaaata aatttttagt ttaggttcag 8401 ttccacttgg gcgaacagca aatcatgact tatcttctaa ataaaatttt agtaagtctt 8461 gtcctggcat attatacatt ccatcgatgt agtcttcaac attaacaact ttaagtccag 8521 caatttgagt taagggtgtt gctctcaatg atttcattaa tggttcaatt tttaatttct 8581 tttcttctgg tttaaaattc aagtttaaag tgaaagtgta atatgcaccc atttctttaa 8641 ataaatcttc taaatagtct actaatgttt tattttgttt tttataaaat caagcagcct 8701 ctgctattaa tatagaagct tgtattccat ctttatctct agctgagtca tcaattacat 8761 atccataact ttcttcataa gcaaaaacaa aatttaatcc gttatcttct tctttagcaa 8821 tttctctacc cattcattta aatccagtta aagtttttac aatattaact ccatattttt 8881 catgagcgat tctatcaccc aaatcacttg ttacaaaact tgaatataga gccggatttt 8941 ttggaatgct atttaagcgt tttagatttg ataattttca atcaattaaa attggtcctg 9001 tttgatttcc atctaatctt acaaaatgac catcatgttt tattgccatt ccaaatctgt 9061 cagcatctgg gtcattcata ataataatat ctgcatcatg tttaatacca tattcaagcg 9121 gtatttttca tgcaggatca aattctggat ttggatttac aacattttta aatgtttcat 9181 cttcaaatgc atgctcttca acctcaataa cgttatatcc tgattcacgt aatatttttg 9241 gggtaaattt agttcctgtt ccattaactg cgctaaaaat aatttttaaa tcttttttag 9301 cttcttgctc ttttttgtag aattctagat cttgagacaa atggcagtat tcatccacaa 9361 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 9421 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 9481 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg //