LOCUS pLKO_TRC011 9560 bp DNA circular 21-AUG-2008 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|488539741| COMMENT VNTDBDATE|491260555| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_TRC011| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/label=5'LTR\Chimeric misc_structure 9391..9508 /vntifkey="88" /label=cPPT rep_origin 2697..3127 /vntifkey="33" /label=F1\Ori misc_feature 5960..6323 /vntifkey="21" /label=gag misc_structure 6438..6841 /vntifkey="88" /label=RRE\(HIV) misc_feature 5856..5993 /vntifkey="21" /label=psi misc_structure 7481..9349 /vntifkey="88" /label=1.9\kb\Stuffer /note="stuffer" misc_feature 523..1242 /vntifkey="21" /label=EGFP misc_feature 1255..1843 /vntifkey="21" /label=WPRE BASE COUNT 2522 a 2247 c 2194 g 2597 t ORIGIN 1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatcccgcca ccatggtgag caagggcgag 541 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 601 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 661 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 721 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 781 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 841 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 901 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 961 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 1021 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 1081 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 1141 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 1201 gccgccggga tcactctcgg catggacgag ctgtacaagt aaggtaccac gcgtaatcaa 1261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 1321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 1381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 1441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 1501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 1561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 1621 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt 1681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 1741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 1801 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcggtacct ttaagaccaa 1861 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 1921 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt 1981 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2041 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2101 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 2161 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 2221 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 2281 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 2341 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc 2401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 2461 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 2521 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 2581 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 2641 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 2701 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 2761 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 2821 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 2881 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 2941 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 3001 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 3061 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 3121 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 3181 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 3241 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 3301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 3361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 3421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 3481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 3541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 3601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 3661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 3721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 3781 ctcgccttga 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accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 4741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 4861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 4921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 5041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 5101 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 5161 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 5221 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 5281 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 5341 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta 5401 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt 5461 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc 5521 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca 5581 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga 5641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 5701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 5761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg 5821 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa 5881 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc 5941 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga 6001 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat 6061 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca 6121 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa 6181 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag 6241 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc 6301 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg 6361 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac 6421 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt 6481 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt 6541 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat 6601 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag 6661 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc 6721 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct 6781 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 6841 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 6901 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 6961 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 7021 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 7081 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 7141 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga 7201 ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt 7261 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa 7321 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt 7381 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt 7441 cttggcttta tatatcttgt ggaaaggacg aaacaccggt gtgttgtaaa tgagcacaca 7501 aaatacacat gctaaaatat tatattctat gacctttata aaatcaacca aaatcttctt 7561 tttaataact ttagtatcaa taattagaat ttttatgttc ctttttgcaa acttttaata 7621 aaaatgagca aaataaaaaa acgctagttt tagtaactcg cgttgttttc ttcaccttta 7681 ataatagcta ctccaccact tgttcctaag cggtcagctc ctgcttcaat cattttttga 7741 gcatcttcaa atgttctaac tccaccagct gctttaacta aagcattgtc tttaacaact 7801 gacttcatta gtttaacatc ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt 7861 ttaacaaatt ctaatccagc ttcaacagct atttcacaag ctttcatgat ttcttctttt 7921 gttaataaac aattttccat aatacattta acaacatgtg atccagctgc tttttttaca 7981 gctttcatgt cttctaaaac taattcataa tttttgtctt ttaatgcacc aatatttaat 8041 accatatcaa tttctgttgc accatcttta attgcttcag aaacttcgaa tgcttttgta 8101 gctgttgtgc atgcacctag aggaaaacct acaacatttg ttattcctac atttgtgcct 8161 tttaataatt ctttacaata gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca 8221 aattcaattg cttcatcaca taattgttta atttcagctt tcgtagcatc ttgttttaat 8281 aatgtgtgat ctatatattt gtttagtttc attttttctc ctatatattc atttttaatt 8341 ttaattcttt aataatttcg tctactttaa ctttagcgtt ttgaacagat tcaccaacac 8401 ctataaaata aatttttagt ttaggttcag ttccacttgg gcgaacagca aatcatgact 8461 tatcttctaa ataaaatttt agtaagtctt gtcctggcat attatacatt ccatcgatgt 8521 agtcttcaac attaacaact ttaagtccag caatttgagt taagggtgtt gctctcaatg 8581 atttcattaa tggttcaatt tttaatttct tttcttctgg tttaaaattc aagtttaaag 8641 tgaaagtgta atatgcaccc atttctttaa ataaatcttc taaatagtct actaatgttt 8701 tattttgttt tttataaaat caagcagcct ctgctattaa tatagaagct tgtattccat 8761 ctttatctct agctgagtca tcaattacat atccataact ttcttcataa gcaaaaacaa 8821 aatttaatcc gttatcttct tctttagcaa tttctctacc cattcattta aatccagtta 8881 aagtttttac aatattaact ccatattttt catgagcgat tctatcaccc aaatcacttg 8941 ttacaaaact tgaatataga gccggatttt ttggaatgct atttaagcgt tttagatttg 9001 ataattttca atcaattaaa attggtcctg tttgatttcc atctaatctt acaaaatgac 9061 catcatgttt tattgccatt ccaaatctgt cagcatctgg gtcattcata ataataatat 9121 ctgcatcatg tttaatacca tattcaagcg gtatttttca tgcaggatca aattctggat 9181 ttggatttac aacattttta aatgtttcat cttcaaatgc atgctcttca acctcaataa 9241 cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt ccattaactg 9301 cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtag aattctcgac 9361 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta 9421 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca 9481 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact 9541 ttggccgcgg ctcgaggggg //